多肽單抗
服務(wù)編號：YKSM-2003
服務(wù)標(biāo)準(zhǔn)：
1.2-5株雜交瘤細(xì)胞株
2.每株細(xì)胞提供2-5 mg抗體
3.抗體ELISA≥80,000
服務(wù)內(nèi)容：
1.多肽合成（10mg，純度〉85%）
2.多肽偶聯(lián)（KLH用于免疫，BSA用于檢測）
3.免疫6只 Bal b/c小鼠，每只小鼠4-5次免疫，小鼠血清Elisa效價達(dá)到1：10000以上
4.取血清效價最高的小鼠的脾細(xì)胞與SP2/0進(jìn)行細(xì)胞融合
5.3-4次亞克隆后得到穩(wěn)定分泌特異性抗體的雜交瘤細(xì)胞株
6.雜交瘤細(xì)胞株擴增/凍存
7.小鼠腹水制備，Protein G純化得到抗體
8.抗體Elisa檢測效價，亞型鑒定
最終交付的材料：
1. 2-5株雜交瘤細(xì)胞株
2. 每株細(xì)胞提供2-5 mg Protein G純化的抗體
3. 1 mg多肽
4. Elisa數(shù)據(jù)報告，亞型鑒定報告，完整項目報告
Peptide → Monoclonal antibody
Service Including:
a.Peptide antigen design and preparation:
Peptide synthesis of up to 18 AA residues (20-30 mg) with a purity > 85%
b.Peptide conjugation:
Peptide conjugation to KLH and BSA
c.Immunization and serum titer test:
Pre-immune bleed
Six Balb/c mice immunization
Serum titer test
d.Fusion and Screening:
Fusion and Screening
Subcloning and cell expansion
e.Antibody production & purification:
Appropriate scale hybridoma cell culture
Purified by protein A affinity chromatography
Customer to provide:
Peptide Sequence
Delivery:
a.2-5hybridoma cell lines;
b.2-5 mg protein A purified mAb per cell line.
c.mAb ELISA titer ≥80,000
Time:
20-26 weeks
