二抗在免疫學(xué)實(shí)驗(yàn)中起著舉足輕重的作用,二抗的選擇也決定了整個(gè)實(shí)驗(yàn)的成敗。實(shí)驗(yàn)中在選擇二抗時(shí)必須注意以下幾點(diǎn):(1) 一抗種屬來(lái)源,主要根據(jù)一抗種屬來(lái)源來(lái)決定確定二抗的類(lèi)型,如一抗是小鼠來(lái)源,那二抗就買(mǎi)抗小鼠的即可(如羊抗小鼠、兔抗小鼠等均可)。
(2)標(biāo)記物的選擇。一般來(lái)講,耦聯(lián)到二抗上的探針主要有酶(辣根過(guò)氧化酶HRP和堿性磷酸酶AP或其衍生物APAAP,PAP),熒光基團(tuán)(FITC, RRX, TR, PE)和生物素。選用哪種探針的二抗主要取決于具體的實(shí)驗(yàn)。對(duì)于western blot和ELISA,常用的二抗是酶標(biāo)二抗,而細(xì)胞或組織標(biāo)記實(shí)驗(yàn)(細(xì)胞免疫化學(xué),組織免疫化學(xué),流式細(xì)胞術(shù))中通常使用熒光基團(tuán)標(biāo)記的二抗,免疫組化中也可以使用辣根過(guò)氧化酶或堿性磷酸酶標(biāo)記的二抗。如果想要更大程度的放大檢測(cè)信號(hào),可以使用biotin/avidin檢測(cè)系統(tǒng)。
友科生物可以提供多種常規(guī)二抗,質(zhì)優(yōu)價(jià)廉,歡迎選購(gòu)。
編號(hào) | 識(shí)別的一抗種屬 | 名稱(chēng) | 應(yīng)用 | 規(guī)格 |
YKGP201H | MOUSE IgG,HRP | Anti-MOUSE IgG (H&L) (GOAT) Antibody Peroxidase Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP201F | MOUSE IgG,FITC | Anti-MOUSE IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | IF | 100ul |
500ul | ||||
1ml | ||||
YKGP201B | MOUSE IgG,BIOTIN | Anti-MOUSE IgG (H&L) (GOAT) Antibody Biotin Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP202H | RABBIT IgG,HRP | Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP202F | RABBIT IgG,FITC | Anti-RABBIT IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | IF | 100ul |
500ul | ||||
1ml | ||||
YKGP202B | RABBIT IgG,BIOTIN | Anti-RABBIT IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP203H | HUMAN IgG,HRP | Anti-HUMAN IgG (H&L) (GOAT) Antibody Peroxidase Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKGP203F | HUMAN IgG,FITC | Anti-HUMAN IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | IF | 100ul |
500ul | ||||
1ml | ||||
YKGP203B | HUMAN IgG,BIOTIN | Anti-HUMAN IgG (H&L) (GOAT) Antibody Fluorescein Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKRP204H | GOAT IgG,HRP | Anti-GOAT IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml | ||||
YKRP204F | GOAT IgG,FITC | Anti-GOAT IgG (H&L) (RABBIT) Antibody Fluorescein Conjugated | IF | 100ul |
500ul | ||||
1ml | ||||
YKRP204B | GOAT IgG,BIOTIN | Anti-GOAT IgG (H&L) (RABBIT) Antibody Fluorescein Conjugated | Elisa/WB/IHC | 100ul |
500ul | ||||
1ml |
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